IL3RA Antibody / CD123

NSJ Bioreagents
Product Code: NSJ-V7468SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7468SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CDLA123-1R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant IL3RA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human tonsil with IL3RA antibody (clone CDLA123-1R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min and allow to cool.~
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IHC staining of FFPE human tonsil with IL3RA antibody (clone CDLA123-1R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min and allow to cool.~

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant IL3RA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD123/IL3RA is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 26-171 was used as the immunogen for the recombinant IL3RA antibody.
Limitation:
This recombinant IL3RA antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P26951