Cadherin 16 Antibody

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NSJ Bioreagents
Product Code: NSJ-V2429SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2429SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDH16/1071
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cadherin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human renal cell carcinoma with Cadherin 16 antibody (clone CDH16/1071). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE rat kidney with Cadherin 16 antibody (clone CDH16/1071). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
3 / 6
Immunofluorescent staining of human kidney tissue with Cadherin 16 antibody (purple, clone CDH16/1071).
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Western blot testing of human kidney lysate with Cadherin 16 antibody (clone CDH16/1071). Expected molecular weight: 90~130 kDa.
5 / 6
Flow cytometry testing of human HEK293 cells with Cadherin 16 antibody (clone CDH16/1071); Red=isotype control, Blue= Cadherin 16 antibody.
6 / 6
SDS-PAGE analysis of purified, BSA-free Cadherin 16 antibody (clone CDH16/1071) as confirmation of integrity and purity.
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IHC testing of FFPE human renal cell carcinoma with Cadherin 16 antibody (clone CDH16/1071). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat kidney with Cadherin 16 antibody (clone CDH16/1071). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human kidney tissue with Cadherin 16 antibody (purple, clone CDH16/1071).
Western blot testing of human kidney lysate with Cadherin 16 antibody (clone CDH16/1071). Expected molecular weight: 90~130 kDa.
Flow cytometry testing of human HEK293 cells with Cadherin 16 antibody (clone CDH16/1071); Red=isotype control, Blue= Cadherin 16 antibody.
SDS-PAGE analysis of purified, BSA-free Cadherin 16 antibody (clone CDH16/1071) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Cadherin 16 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the Cadherin 16 antibody.
Limitation:
This Cadherin 16 antibody is available for research use only.
Localization:
Cell surface with some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
O75309