MUC2 Antibody / Mucin 2

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This antibody recognizes a single glycoprotein of 520kDa, identified as MUC2 (Mucin-2). The MUC2 antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine and colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

4583

Amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT, from human MUC2 / Mucin-2 protein, were used as the immunogen for this antibody.

This MUC2 antibody is available for research use only.

Cytoplasmic and cell surface

Protein G affinity chromatography