Cyclin D1 Antibody / CCND1
| Code | Size | Price |
|---|
| NSJ-V2365PE-100T | 100 Tests | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: CCND1/809
Regulatory Status: RUO
Target Species:
- Human
- Monkey
- Mouse
- Rat
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive store in the dark.
Documents
Further Information
Application Details:
Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence: 1:50-1:100 for 30 minutes at RT
Application Note:
The dilution stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CCND1/809 mAb to be titered up or down for optimal performance.
Description:
This antibody recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. The antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Format:
PE Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
595
Immunogen:
Human recombinant full length Cyclin D1 protein was used as the immunogen.
Limitation:
This Cyclin D1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography