Caldesmon / CAD Antibody HMW

NSJ Bioreagents
Product Code: NSJ-V7252SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7252SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CALD1/1424R
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Caldesmon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone CALD1/1424R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).~
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IHC testin

Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone CALD1/1424R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).~
IHC testin

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Titering of the recombinant Caldesmon antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.
Limitation:
This recombinant Caldesmon antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
Q05682