CA9 Antibody / Carbonic Anhydrase IX

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NSJ Bioreagents
Product Code: NSJ-V8463SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8463SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: CA9/3406
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa.
2 / 6
Flow cytometry testing of FPA fixed human U-87 MG cells with CA9 antibody; Red=isotype control, Blue= CA9 antibody.
3 / 6
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CA9 antibody. These results demonstrate the foremost specificity of the CA9/3406 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
6 / 6
SDS-PAGE analysis of purified, BSA-free CA9 antibody as confirmation of integrity and purity.
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Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa.
Flow cytometry testing of FPA fixed human U-87 MG cells with CA9 antibody; Red=isotype control, Blue= CA9 antibody.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CA9 antibody. These results demonstrate the foremost specificity of the CA9/3406 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CA9 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the CA9 antibody should be determined by the researcher.
Description:
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 314-410 from the human protein was used as the immunogen for the CA9 antibody.
Limitation:
This CA9 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q16790