CD68 Antibody

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NSJ Bioreagents
Product Code: NSJ-V2074SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2074SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: C68/684
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684).
2 / 6
Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.
3 / 6
Immunofluorescent staining of fixed human U-87 MG cells with CD68 antibody (clone C68/684, green) and Reddot nuclear stain (red).
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Flow cytometry testing of fixed human U-87 MG cells with CD63 antibody (clone C68/684); Red=isotype control, Blue= CD63 antibody.
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Western blot testing of human spleen lysate with CD68 antibody (clone C68/684). Expected molecular weight: 37-110 kDa depending on glycosylation level.
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SDS-PAGE analysis of purified, BSA-free CD68 antibody (clone C68/684) as confirmation of integrity and purity.
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IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684).
Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.
Immunofluorescent staining of fixed human U-87 MG cells with CD68 antibody (clone C68/684, green) and Reddot nuclear stain (red).
Flow cytometry testing of fixed human U-87 MG cells with CD63 antibody (clone C68/684); Red=isotype control, Blue= CD63 antibody.
Western blot testing of human spleen lysate with CD68 antibody (clone C68/684). Expected molecular weight: 37-110 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD68 antibody (clone C68/684) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
968
Immunogen:
The subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody.
Limitation:
This CD68 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography