NSJ Bioreagents

Cytokeratin 5/8 Antibody

Product Code:
 
NSJ-V3059IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C-50
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.~
2 / 2
IHC analysis of formalin-fixed, paraffin-e
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IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.~
IHC analysis of formalin-fixed, paraffin-e

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NSJ-V3059IHC-7ML7 ml£534.00
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
Limitation:
This Cytokeratin 5/8 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P13647, P05787

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