Cytokeratin Antibody Multi 4/5/6/8/10/13/18

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NSJ Bioreagents
Product Code: NSJ-V2331SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2331SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: C11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).
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IHC testing of FFPE rat oviduct and Multi Cytokeratin antibody (clone C11).
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SDS-PAGE analysis of purified, BSA-free Multi Cytokeratin antibody (clone C11) as confirmation of integrity and purity.
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IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).
IHC testing of FFPE rat oviduct and Multi Cytokeratin antibody (clone C11).
SDS-PAGE analysis of purified, BSA-free Multi Cytokeratin antibody (clone C11) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3851 (CK4); 3852 (CK5); 3853 (CK6); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)
Immunogen:
A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
Limitation:
This Multi Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography