Bromodeoxyuridine / BrdU Antibody

NSJ Bioreagents
Product Code: NSJ-V2321CF488
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2321CF488-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: BRD469
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the Bromodeoxyuridine antibody at 2-8oC protected from light.

Documents

Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
Application Note:
Optimal dilution of the Bromodeoxyuridine antibody should be determined by the researcher.
Description:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Limitation:
This Bromodeoxyuridine antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography