CD45RB Antibody (B-cell marker)

Flow Cytometry: 5ul/test/million cells in 100ul (or 5ul per 100ul of whole blood),Immunofluorescence: 1:50-1:100

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. antibody to CD45R is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

PE Conjugate

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

5788

The non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V) was used as the immunogen for this antibody.

This CD45RB antibody is available for research use only.

Cell surface and cytoplasmic

Protein G affinity chromatography