Granulocyte Marker Antibody

NSJ Bioreagents
Product Code: NSJ-V2339IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2339IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: BM-2
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Granulocyte Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of tonsil tissue with Granulocyte Marker antibody (clone BM-2). Note specific cytoplasmic staining.~
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SDS-PAGE Analysis of Purified, BSA-Free Granulocyte Marker Antibody (clone BM-2). Confirmation of Integrity and Purity of t

IHC staining of tonsil tissue with Granulocyte Marker antibody (clone BM-2). Note specific cytoplasmic staining.~
SDS-PAGE Analysis of Purified, BSA-Free Granulocyte Marker Antibody (clone BM-2). Confirmation of Integrity and Purity of t

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte Marker antibody.
Limitation:
This Granulocyte Marker antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography