Chromogranin A Antibody

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NSJ Bioreagents
Product Code: NSJ-V3720SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3720SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: bGRAN2R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC analysis of FFPE human parathyroid gland stained with recombinant Chromogranin A antibody (clone bGRAN2R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min and allow to cool at RT for 20 min before testing.
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IHC analysis of FFPE human parathyroid gland stained with recombinant Chromogranin A antibody (clone bGRAN2R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min and allow to cool at RT for 20 min before testing.

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Further Information

Application Details:
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Chromogranin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Chromogranin A or parathyroid secretory protein 1 (gene name CHGA) is a member of the granin family of neuroendocrine secretory proteins, i.e., it is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A is the precursor to several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human protein was used as the immunogen for the recombinant Chromogranin A antibody.
Limitation:
This recombinant Chromogranin A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P10645