CD3 Antibody

NSJ Bioreagents
Product Code: NSJ-V2379SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2379SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: B-B12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone B-B12) and Reddot nuclear stain (red).~
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Flow cytometry testing of human Jurkat cells with CD3 antibody (clone B-B12); Red=isotype control, Blue= CD3 an

Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone B-B12) and Reddot nuclear stain (red).~
Flow cytometry testing of human Jurkat cells with CD3 antibody (clone B-B12); Red=isotype control, Blue= CD3 an

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.
Description:
CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human T cell leukemia cells were used as immunogen for the CD3 antibody.
Limitation:
The CD3 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P07766