VTCN1 Antibody / B7H4

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NSJ Bioreagents
Product Code: NSJ-V8175SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8175SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: B7H4/1788
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the B7-H4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788, green) and Reddot nuclear stain (red).
2 / 5
Flow cytometry testing of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788); Red=isotype control, Blue= B7-H4 antibody.
3 / 5
IHC staining of FFPE human testicular carcinoma with B7-H4 antibody (clone B7H4/1788). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human HCT-116 cell lysate with B7-H4 antibody. Predicted molecular weight: ~31 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free B7-H4 antibody (clone B7H4/1788) as confirmation of integrity and purity.
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Immunofluorescent staining of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788, green) and Reddot nuclear stain (red).
Flow cytometry testing of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788); Red=isotype control, Blue= B7-H4 antibody.
IHC staining of FFPE human testicular carcinoma with B7-H4 antibody (clone B7H4/1788). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HCT-116 cell lysate with B7-H4 antibody. Predicted molecular weight: ~31 kDa.
SDS-PAGE analysis of purified, BSA-free B7-H4 antibody (clone B7H4/1788) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein was used as the immunogen for this B7-H4 antibody.
Limitation:
This B7-H4 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q7Z7D3