Arginase 1 Antibody Cocktail

Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the Arginase 1 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody cocktail.

This Arginase 1 antibody cocktail is available for research use only.

Cytoplasmic

Protein G affinity chromatography

P05089