Amyloid beta Antibody / APP

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NSJ Bioreagents
Product Code: NSJ-V8733SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8733SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: APP/3345
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Amyloid Beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Immunofluorescent staining of PFA-fixed human U-87 MG cells with Amyloid Beta antibody (clone APP/3345, green) and Reddot nuclear stain (red).
2 / 4
IHC staining of FFPE human brain with Amyloid Beta antibody (clone APP/3345). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Amyloid Beta antibody (clone APP/3345). These results demonstrate the foremost specificity of the APP/3345 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free Amyloid Beta antibody (clone APP/3345) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human U-87 MG cells with Amyloid Beta antibody (clone APP/3345, green) and Reddot nuclear stain (red).
IHC staining of FFPE human brain with Amyloid Beta antibody (clone APP/3345). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Amyloid Beta antibody (clone APP/3345). These results demonstrate the foremost specificity of the APP/3345 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Amyloid Beta antibody (clone APP/3345) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Amyloid Beta antibody should be determined by the researcher.
Description:
Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the /A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 578-680 from the human protein was used as the immunogen for the Amyloid Beta antibody.
Limitation:
This Amyloid Beta antibody is available for research use only.
Localization:
Membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P05067