Annexin A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7838SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7838SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: ANXA1/3566
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 9
Immunofluorescent staining of PFA-fixed human HeLa cells with Annexin A1 antibody (green, clone ANXA1/3566) and Reddot nuclear stain (red).
2 / 9
IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human colon carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human prostate carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
6 / 9
Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/3566). Predicted molecular weight ~38 kDa.
7 / 9
Flow cytometry testing of PFA-fixed human HeLa cells with Annexin A1 antibody (clone ANXA1/3566); Red=isotype control, Blue= Annexin A1 antibody.
8 / 9
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Annexin A1 antibody (clone ANXA1/3566). These results demonstrate the foremost specificity of the ANXA1/3566 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
9 / 9
SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/3566) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells with Annexin A1 antibody (green, clone ANXA1/3566) and Reddot nuclear stain (red).
IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/3566). Predicted molecular weight ~38 kDa.
Flow cytometry testing of PFA-fixed human HeLa cells with Annexin A1 antibody (clone ANXA1/3566); Red=isotype control, Blue= Annexin A1 antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Annexin A1 antibody (clone ANXA1/3566). These results demonstrate the foremost specificity of the ANXA1/3566 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/3566) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.
Limitation:
This Annexin A1 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P04083