Placental Alkaline Phosphatase Antibody / ALPP

ELISA (order BSA/sodium azide-free format for coating)

Optimal dilution of the Placental Alkaline Phosphatase antibody should be determined by the researcher.

PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant full-length human ALPP protein was used as the immunogen for the Placental Alkaline Phosphatase antibody.

This Placental Alkaline Phosphatase antibody is available for research use only.

Cytoplasmic and cell surface

Protein G affinity chromatography

P05187