Placental Alkaline Phosphatase Antibody / ALPP
| Code | Size | Price |
|---|
| NSJ-V2514SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: ALP/870
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Placental Alkaline Phosphatase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2),Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the Placental Alkaline Phosphatase antibody should be determined by the researcher.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ALPP protein was used as the immunogen for the Placental Alkaline Phosphatase antibody.
Limitation:
This Placental Alkaline Phosphatase antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P05187