ALDH1A1 Antibody / Acetaldehyde dehydrogenase 1

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NSJ Bioreagents
Product Code: NSJ-V7764SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7764SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ALDH1A1/1381
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the ALDH1A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
IHC staining of FFPE human testicular carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human colon carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 5
Western blot testing of human liver lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.
4 / 5
Western blot testing of human K562 (left) and lung (right) lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free ALDH1A1 antibody (clone ALDH1A1/1381) as confirmation of integrity and purity.
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IHC staining of FFPE human testicular carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human liver lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.
Western blot testing of human K562 (left) and lung (right) lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.
SDS-PAGE analysis of purified, BSA-free ALDH1A1 antibody (clone ALDH1A1/1381) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A human recombinant partial ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.
Limitation:
This ALDH1A1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P00352