dsDNA Antibody / Double Stranded DNA
Code | Size | Price |
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NSJ-V3099SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG3 kappa
Antibody Clonality: Monoclonal
Antibody Clone: AE-2
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
Application Note:
Optimal dilution of the dsDNA antibody should be determined by the researcher.
Description:
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
Limitation:
This dsDNA antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography