Pan Cytokeratin Antibody Cocktail (Acidic + Basic)
Code | Size | Price |
---|
NSJ-V2330FITC-100T | 100 Tests | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa + Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: AE-1/AE-3
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Store the Pan Cytokeratin antibody at 2-8oC protected from light.
Documents
Further Information
Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
Application Note:
Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.
Description:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Format:
FITC Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Human epidermal keratin was used as the immunogen for this Pan Cytokeratin antibody.
Limitation:
This Pan Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography