Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

PE Conjugate

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

3848 (K1); 3850 (K3); 3851 (K4); 3852 (K5); 3853 (K6A); 3856 (K8);3858 (K10); 3861 (K14); 3866 (K15); 3868 (K16); 3880 (K19)

Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

This pan Cytokeratin antibody AE1 + AE3 is available for research use only.

Cytoplasmic

Protein G affinity chromatography