HSP60 Antibody / Heat Shock Protein 60

NSJ Bioreagents
Product Code: NSJ-V8546SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8546SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: AE-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
Immunofluorescent staining of PFA-fixed human HeLa cells with HSP60 antibody (clone AE-1, green) and Reddot nuclear stain (red).~
2 / 2
IHC staining of FFPE human renal cell carcinoma with HSP60 antibody (clone AE-1). HIER: boil tissue section

Immunofluorescent staining of PFA-fixed human HeLa cells with HSP60 antibody (clone AE-1, green) and Reddot nuclear stain (red).~
IHC staining of FFPE human renal cell carcinoma with HSP60 antibody (clone AE-1). HIER: boil tissue section

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the HSP60 antibody should be determined by the researcher.
Description:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the HSP60 antibody.
Limitation:
This HSP60 antibody is available for research use only.
Localization:
Cytoplasm (mitochondria)
Purity:
Protein G affinity chromatography
Uniprot #:
P10809