Alpha 1 Antitrypsin Antibody / AAT / SERPINA1

NSJ Bioreagents
Product Code: NSJ-V8013SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8013SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: AAT/3167R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human breast with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human samples with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R) as confirmation of integrity and purity.

IHC staining of FFPE human breast with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
SDS-PAGE analysis of purified, BSA-free recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human Alpha-1-Antitrypsin (AAT) partial protein was used as the immunogen for this recombinant Alpha 1 Antitrypsin antibody.
Limitation:
This recombinant Alpha 1 Antitrypsin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P01009