CDK1 Antibody
| Code | Size | Price |
|---|
| NSJ-V3748SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: A17.1.1
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
- Inhibition Assay
- Kinase Assay
- Western Blot (WB)
Storage:
Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence: 1-2ug/ml,Immunoprecipitation,Kinase assay,Western blot,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CDC2 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a 34kDa protein (cdk1), identified as Cdk1/Cdc2/p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This mAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
983
Immunogen:
A partial protein (the C-terminal two-thirds of Xenopus CDC2) was used as the immunogen for this CDC2 antibody.
Limitation:
This CDC2 antibody is available for research use only.
Localization:
Nuclear and Cytoplasmic
Purity:
Protein G purified antibody
Uniprot #:
P06493