MART-1 / Melan-A Antibody
Code | Size | Price |
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NSJ-V2121SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: A103
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Due to differences in protocols and secondary antibody used, the Melan-A antibody may require titration for optimal performance.
1. FFPE staining is enhanced by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Antibody A103 is specific for Melan-A (likely an abbreviation for 'melanocyte antigen'), also called MART-1, a unique antigen on melanoma cells recognized by CTLs (cytotoxic T lymphocytes), making it useful as a melanocyte differentiation marker. It is commonly used to identify metastatic tumors of melanocytic origin, as well as perivascular epithelioid cell tumors. Unlike another commonly used Melan-A / MART-1 antibody, M2-7C10, A103 also stains steroid hormone producing cells, making it valuable in identifying adrenocortical carcinomas.
Note: this protein was identified in 1994 by two different labs, one naming it MART-1 (melanoma antigen recognized by T-cells) and the other Melan-A. Both names are commonly used in literature, but clone A103 antibody is generally referred to as being anti-Melan-A.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
2315
Immunogen:
Recombinant human protein was used as the immunogen for the Melan-A antibody.
Limitation:
This Melan-A antibody is available for research use only, not for diagnostic or therapeutic testing.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography