CD73 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54319
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54319-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 974CT7.5.3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD73 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human WiDr cell lysate with CD73 antibody. Predicted molecular weight: 65-70 kDa.
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Western blot testing of human MDA-MB-231 cell lysate with CD73 antibody. Predicted molecular weight: 65-70 kDa.
3 / 4
IHC testing of FFPE human kidney tissue with CD73 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human kidney tissue with CD73 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human WiDr cell lysate with CD73 antibody. Predicted molecular weight: 65-70 kDa.
Western blot testing of human MDA-MB-231 cell lysate with CD73 antibody. Predicted molecular weight: 65-70 kDa.
IHC testing of FFPE human kidney tissue with CD73 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with CD73 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details:
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Format:
Purified
Formulation:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the CD73 antibody.
Limitation:
This CD73 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity
Uniprot #:
P21589