AIFM1 Antibody / AIF / Apoptosis-Inducing Factor

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NSJ Bioreagents
Product Code: NSJ-F54034
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54034-0.1ML0.1 ml£502.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 8H1-B10-A12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the AIF antibody at -20oC.

Images

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ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using AIF antibody at 1:200.~
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Western blot testing of human 1) HeLa, 2) Ramos, 3) HepG2, 4) MCF7 and 5) Jurkat cell lysates using AIF antibody at 1:1000. Predicted molecula
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ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using AIF antibody at 1:200.~
Western blot testing of human 1) HeLa, 2) Ramos, 3) HepG2, 4) MCF7 and 5) Jurkat cell lysates using AIF antibody at 1:1000. Predicted molecula

Documents

Further Information

Application Details:
Western blot: 1:1000,ICC/Immunofluorescence: 1:200
Application Note:
The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant partial protein was used as the immunogen for this AIF antibody.
Limitation:
This AIF antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
O95831