NGFR Antibody

NSJ Bioreagents
Product Code: NSJ-F53510
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F53510-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b k
Antibody Clonality: Monoclonal
Antibody Clone: 8G3G10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the NGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Fluorescent confocal image of SY5Y cells stained with NGFR antibody. Alexa Fluor 488 secondary Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
2 / 3
Western blot analysis of NGFR antibody and mouse heart tissue lysate
3 / 3
NGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.

Fluorescent confocal image of SY5Y cells stained with NGFR antibody. Alexa Fluor 488 secondary Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
Western blot analysis of NGFR antibody and mouse heart tissue lysate
NGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.

Documents

Further Information

Application Details:
Immunofluorescence: 1:100,Western blot: 1:500-1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
Format:
Purified
Formulation:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This NGFR antibody was produced from mouse immunized with NGFR recombinant protein.
Limitation:
This NGFR antibody is available for research use only.
Purity:
Purified
Uniprot #:
P08138