CD147 / Emmprin / Basigin Antibody

Inhibits homotypic aggregation, adhesion to matrix proteins, and migation through matrigel (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

682

Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.

This CD147 antibody is available for research use only.

Cell surface, cytoplasmic

Protein G purified CD147 antibody