Phosphotyrosine Antibody

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NSJ Bioreagents
Product Code: NSJ-F54032
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54032-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 7B4-D9-H10
Regulatory Status: RUO
Target Species: Species Independent
Application: Western Blot (WB)
Storage:
Store the Phosphotyrosine antibody at -20oC.

Images

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Western blot analysis of extracts from A549 cells, untreated and treated with Epidermal Growth Factor (10ng/ml, 15min), using Phosphotyrosine antibody. The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.
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Western blot analysis of extracts from A549 cells, untreated and treated with Epidermal Growth Factor (10ng/ml, 15min), using Phosphotyrosine antibody. The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.

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Further Information

Application Details:
Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation.
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A phospho-tyrosine containing peptide was used as the immunogen for this Phosphotyrosine antibody.
Limitation:
This Phosphotyrosine antibody is available for research use only.
Purity:
Protein G affinity