SIRT6 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F54008
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54008-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6C9-D10-D3
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Non-Human Primates
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the SIRT6 antibody at -20oC.

Images

1 / 3
Western blot testing of human Jurkat, MCF7, HCT116, K562, monkey COS7, mouse C2C12, mouse NIH3T3 and human HeLa cell lysates using SIRT6 antibody at 1:500. Predicted molecular weight ~42 kDa.
2 / 3
ICC/IF testing of HeLa cells, fixed by 4% paraformaldehyde, with SIRT6 antibody at 1:100.
3 / 3
Immunoprecipitation of SIRT6 from HeLa cell lysate using SIRT6 antibody. The precipitate was subsequently immunoblotted with the same mAb.
❮❯

Western blot testing of human Jurkat, MCF7, HCT116, K562, monkey COS7, mouse C2C12, mouse NIH3T3 and human HeLa cell lysates using SIRT6 antibody at 1:500. Predicted molecular weight ~42 kDa.
ICC/IF testing of HeLa cells, fixed by 4% paraformaldehyde, with SIRT6 antibody at 1:100.
Immunoprecipitation of SIRT6 from HeLa cell lysate using SIRT6 antibody. The precipitate was subsequently immunoblotted with the same mAb.

Documents

Further Information

Application Details:
Western blot: 1:500,ICC/Immunofluorescence: 1:100,Immunoprecipitation: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the SIRT6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant protein was used as the immunogen for this SIRT6 antibody.
Limitation:
This SIRT6 antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
Q8N6T7