FASN Antibody / Fatty acid synthase

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NSJ Bioreagents
Product Code: NSJ-F54047
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54047-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6B9-H12-E7
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the FASN antibody at -20oC.

Images

1 / 3
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using FASN antibody at 1:200.
2 / 3
Western blot testing of 1) human HeLa, 2) rat C6, 3) NIH3T3, 4) monkey CHO-K1 and 5) MCF7 cell lysates using FASN antibody at 1:500. Expected molecular weight ~270 kDa.
3 / 3
Immunoprecipitation of FASN from CHO-K1 cell lysate, and subsequent western blot testing, using FASN antibody.
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ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using FASN antibody at 1:200.
Western blot testing of 1) human HeLa, 2) rat C6, 3) NIH3T3, 4) monkey CHO-K1 and 5) MCF7 cell lysates using FASN antibody at 1:500. Expected molecular weight ~270 kDa.
Immunoprecipitation of FASN from CHO-K1 cell lysate, and subsequent western blot testing, using FASN antibody.

Documents

Further Information

Application Details:
Western blot: 1:500,ICC/Immunofluorescence: 1:200,Immunoprecipitation: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the FASN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Limitation:
This Fatty Acid Synthase antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P49327