CD138 Antibody

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NSJ Bioreagents
Product Code: NSJ-F53713
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F53713-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM
Antibody Clonality: Monoclonal
Antibody Clone: 587CT7.3.6.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the CD138 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
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CD138 antibody flow cytometric analysis of T47D cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
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CD138 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
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Confocal immunofluorescent analysis of CD138 antibody with U266 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Confocal immunofluorescent analysis of CD138 antibody with T47D cells followed by secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
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Confocal immunofluorescent analysis of CD138 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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CD138 antibody western blot analysis in HepG2 lysate. Predicted molecular weight: 20-95 kDa depending on glycosylation level.
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CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
CD138 antibody flow cytometric analysis of T47D cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
CD138 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
Confocal immunofluorescent analysis of CD138 antibody with U266 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of CD138 antibody with T47D cells followed by secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of CD138 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
CD138 antibody western blot analysis in HepG2 lysate. Predicted molecular weight: 20-95 kDa depending on glycosylation level.

Documents

Further Information

Application Details:
Western blot: 1:500-1:8000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the CD138 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD138 is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The Syndecan-1/CD138 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins.
Format:
Ascites
Formulation:
In ascites with 0.09% sodium azide
Immunogen:
A portion of amino acids 210-238 from the human protein was used as the immunogen for this CD138 antibody.
Limitation:
This CD138 antibody is available for research use only.
Purity:
Ascites
Uniprot #:
P18827