Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54035
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54035-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4F8-A5-H5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the Vimentin antibody at -20oC.

Images

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Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
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Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
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ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.
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Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.

Documents

Further Information

Application Details:
Western blot: 1:1000,ICC/Immunofluorescence: 1:800,Immunoprecipitation: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant partial protein was used as the immunogen for this Vimentin antibody.
Limitation:
This Vimentin antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P08670