Testosterone Antibody

NSJ Bioreagents
Product Code: NSJ-V3056SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3056SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 4E1G2
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Radioimmunoassay (RIA)
Storage:
Store the Testosterone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human testicular carcinoma with Testosterone antibody (clone 4E1G2). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
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SDS-PAGE Analysis of Purified

IHC testing of FFPE human testicular carcinoma with Testosterone antibody (clone 4E1G2). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAGE Analysis of Purified

Documents

Further Information

Application Details:
ELISA (order BSA/sodium azide-free format for coating),Radioimmnoassay (RIA),Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Testosterone antibody should be determined by the researcher.
Description:
This mAb is highly specific to testosterone. Its affinity constant for testosterone is ~10^10 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Testosterone 3 CMO conjugated to BSA was used as the immunogen for the Testosterone antibody.
Limitation:
This Testosterone antibody is available for research use only.
Localization:
Secreted and cytoplasmic
Purity:
Protein G affinity chromatography