E-Cadherin Antibody / CDH1

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NSJ Bioreagents
Product Code: NSJ-V3232SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3232SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 4A2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (green, clone 4A2) and Reddot nuclear stain (red).
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IHC testing of FFPE human skin with E-Cadherin antibody (clone 4A2). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone 4A2) as confirmation of integrity and purity.
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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (green, clone 4A2) and Reddot nuclear stain (red).
IHC testing of FFPE human skin with E-Cadherin antibody (clone 4A2). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
SDS-PAGE analysis of purified, BSA-free E-Cadherin antibody (clone 4A2) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
Description:
Recognizes a protein of 80-120kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Limitation:
This E-Cadherin antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Uniprot #:
P12830