FAK Antibody / Focal Adhesion Kinase

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NSJ Bioreagents
Product Code: NSJ-F54023
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54023-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3D8-H11-E12
Regulatory Status: RUO
Target Species:
  • Human
  • Non-Human Primates
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the FAK antibody at -20oC.

Images

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Western blot testing of monkey COS7, human HUVEC, human A549 and human HeLa cell lysates with FAK antibody at 1:1000. FAK has numerous isoforms and is routinely observed in western blot between 80~125 kDa.
2 / 3
ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with FAK antibody at 1:200.
3 / 3
Immunoprecipitation and western blot of HeLa cell lysate using the FAK antibody.
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Western blot testing of monkey COS7, human HUVEC, human A549 and human HeLa cell lysates with FAK antibody at 1:1000. FAK has numerous isoforms and is routinely observed in western blot between 80~125 kDa.
ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with FAK antibody at 1:200.
Immunoprecipitation and western blot of HeLa cell lysate using the FAK antibody.

Documents

Further Information

Application Details:
Western blot: 1:1000,ICC/Immunofluorescence: 1:200,Immunoprecipitation: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the FAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Focal Adhesion Kinase, or FAK, is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant partial protein was used as the immunogen for this FAK antibody.
Limitation:
This FAK antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
Q05397