Podocalyxin Antibody / PODXL

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NSJ Bioreagents
Product Code: NSJ-V2227IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2227IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 3D3
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Podocalyxin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human placenta with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human angiosarcoma with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Podocalyxin antibody (clone 3D3) as confirmation of integrity and purity.
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IHC staining of FFPE human placenta with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human angiosarcoma with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Podocalyxin antibody (clone 3D3) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.
Description:
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
5420
Immunogen:
A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
Limitation:
This Podocalyxin antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography