MMP2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54029
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54029-0.1ML0.1 ml£502.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3A8-C1-E8
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the MMP2 antibody at -20oC.

Images

1 / 3
ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with MMP2 antibody at 1:50. DAPI was used to stain nuclei (blue).
2 / 3
Western blot testing of A) transfected (human MMP2, partial) and B) untransfected CHO-K1 cell lysate with MMP2 antibody at 1:2000. Expected molecular weight of the partial protein: ~30 kDa.
3 / 3
Western blot testing of mouse Raw264.7 cell lysate with MMP2 antibody at 1:200. Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).
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ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with MMP2 antibody at 1:50. DAPI was used to stain nuclei (blue).
Western blot testing of A) transfected (human MMP2, partial) and B) untransfected CHO-K1 cell lysate with MMP2 antibody at 1:2000. Expected molecular weight of the partial protein: ~30 kDa.
Western blot testing of mouse Raw264.7 cell lysate with MMP2 antibody at 1:200. Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).

Documents

Further Information

Application Details:
Western blot: 1:1000,ICC/Immunofluorescence: 1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant protein was used as the immunogen for this MMP2 antibody.
Limitation:
This MMP2 antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P08253