Cig2 Antibody
| Code | Size | Price |
|---|
| NSJ-V8291SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 3A11/5
Regulatory Status: RUO
Target Species: Schizosaccharomyces species (bacteria)
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Store the Cig2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Bacterial S. pombe Cig2 purified protein was used as the immunogen for the Cig2 antibody.
Limitation:
This Cig2 antibody is available for research use only.
Purity:
Protein G affinity chromatography