EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V2187SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2187SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 323/A3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of PFA-fixed human MCF7 cells with EpCAM antibody (clone 323/A3); Red=isotype control, Blue= EpCAM antibody.
2 / 3
Immunofluorescent testing of FFPE HT29 cells with EpCAM antibody (clone 323/A3). Green = EpCAM, red = F-actin and blue = DAPI (nuclear).
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IHC testing of FFPE human colon carcinoma with EpCAM antibody.
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Flow cytometry testing of PFA-fixed human MCF7 cells with EpCAM antibody (clone 323/A3); Red=isotype control, Blue= EpCAM antibody.
Immunofluorescent testing of FFPE HT29 cells with EpCAM antibody (clone 323/A3). Green = EpCAM, red = F-actin and blue = DAPI (nuclear).
IHC testing of FFPE human colon carcinoma with EpCAM antibody.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
4072
Immunogen:
MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography