Phospho-ERK1/2 Antibody (Thr202/Tyr204)

NSJ Bioreagents
Product Code: NSJ-F54044
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54044-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2E8-2F6-C12
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store the phospho-ERK1/2 antibody at -20oC.

Images

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Western blot testing of EGF-treated and untreated A549 cells and PDGF-treated and untreated NIH3T3 cells using phospho-ERK1/2 antibody at 1:500 (upper) and a non-phospho-specific ERK1/2 antibody at 1:500 (lower).

Western blot testing of EGF-treated and untreated A549 cells and PDGF-treated and untreated NIH3T3 cells using phospho-ERK1/2 antibody at 1:500 (upper) and a non-phospho-specific ERK1/2 antibody at 1:500 (lower).

Documents

Further Information

Application Details:
Western blot: 1:500
Application Note:
The stated application concentrations are suggested starting points. Titration of the phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A synthetic peptide corresponding to the amino acids surrounding pThr202 and pTyr204 of human ERK1/2 was used as the immunogen for this phospho-ERK1/2 antibody.
Limitation:
This phospho-ERK1/2 antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P27361, P28482