Clostridium difficile Toxin A Antibody

ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml

Optimal dilution of the antibody should be determined by the researcher.

This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

C. difficile Toxin A was used as the immunogen for the Clostridium difficile Toxin A antibody.

This Clostridium difficile Toxin A antibody is available for research use only.

Protein G affinity chromatography

P16154