Human Nuclear Antigen Antibody

NSJ Bioreagents
Product Code: NSJ-V2345CF488
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2345CF488-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 235-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the Human Nuclear Antigen antibody at 2-8oC protected from light.

Images

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Intracellular flow cytometry testing of human 293T cells with Human Nuclear Antigen antibody; Black=cells alone, Gray=isotype control, Green= Human Nuclear Antigen antibody.

Intracellular flow cytometry testing of human 293T cells with Human Nuclear Antigen antibody; Black=cells alone, Gray=isotype control, Green= Human Nuclear Antigen antibody.

Documents

Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the Human Nuclear Antigen antibody should be determined by the researcher.
Description:
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.
Limitation:
This Human Nuclear Antigen antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography