MUC5AC Antibody

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NSJ Bioreagents
Product Code: NSJ-V2739SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2739SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2-11M1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the MUC5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone 2-11M1). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone 2-11M1). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Details:
ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the MUC5AC antibody should be determined by the researcher.
Description:
This mAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. It has been found that treating the testing sample with Papain or mercaptoethanol will destroy the M1 epitope for the 2-11M1 antibody (Ref. 1; Cancer Research 46.8 (1986): 3983-3989.)
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for the MUC5AC antibody.
Limitation:
This MUC5AC antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P98088