NSE Antibody / Neuron Specific Enolase

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NSJ Bioreagents
Product Code: NSJ-F54039
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54039-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse lgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1H8-B4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Aliquot the NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) rat brain and 2) mouse brain lysates using NSE antibody at 1:500. Predicted molecular weight ~47 kDa.
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Western blot testing of human 1) SHSY-5Y and 2) U87-MG cell lysates using NSE antibody at 1:500. Predicted molecular weight ~47 kDa.
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Observed NSE / Neuron Specific Enolase levels in CHO-K1 cell lysates transfected with NSE at different dilution. Pairing: <a href=../tds/neuron-specific-enolase-antibody-1g7-b4-f54038>1G7 (capture)</a> and 1H8 (detect).
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Western blot testing of 1) rat brain and 2) mouse brain lysates using NSE antibody at 1:500. Predicted molecular weight ~47 kDa.
Western blot testing of human 1) SHSY-5Y and 2) U87-MG cell lysates using NSE antibody at 1:500. Predicted molecular weight ~47 kDa.
Observed NSE / Neuron Specific Enolase levels in CHO-K1 cell lysates transfected with NSE at different dilution. Pairing: <a href=../tds/neuron-specific-enolase-antibody-1g7-b4-f54038>1G7 (capture)</a> and 1H8 (detect).

Documents

Further Information

Application Details:
Western blot: 1:500,ELISA: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the NSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Neuron Specific Enolase (NSE, Enolase-2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Format:
Purified
Formulation:
Antibody in 1X PBS with 0.03% ProClin 300
Immunogen:
A synthetic peptide corresponding to part of the human protein was used as the immunogen for the NSE antibody.
Limitation:
This NSE antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P09104