NSE Antibody / Neuron Specific Enolase

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NSJ Bioreagents
Product Code: NSJ-F54038
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54038-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse lgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1G7-B4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Aliquot the Neuron Specific Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) SHSY-5Y and 2) U87-MG cell lysates using Neuron Specific Enolase antibody at 1:1000. Predicted molecular weight ~47 kDa.
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Western blot testing of 1) rat brain and 2) mouse brain lysates using Neuron Specific Enolase antibody at 1:1000. Predicted molecular weight ~47 kDa.
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Observed NSE / Neuron Specific Enolase levels in CHO-K1 cell lysates transfected with Neuron Specific Enolase at different dilution. Pairing: 1G7 (capture) and <a href=../tds/nse-antibody-1h8-b4-f54039>1H8 (detect)</a>.
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Western blot testing of human 1) SHSY-5Y and 2) U87-MG cell lysates using Neuron Specific Enolase antibody at 1:1000. Predicted molecular weight ~47 kDa.
Western blot testing of 1) rat brain and 2) mouse brain lysates using Neuron Specific Enolase antibody at 1:1000. Predicted molecular weight ~47 kDa.
Observed NSE / Neuron Specific Enolase levels in CHO-K1 cell lysates transfected with Neuron Specific Enolase at different dilution. Pairing: 1G7 (capture) and <a href=../tds/nse-antibody-1h8-b4-f54039>1H8 (detect)</a>.

Documents

Further Information

Application Details:
Western blot: 1:1000,ELISA: suitable
Application Note:
The stated application concentrations are suggested starting points. Titration of the Neuron Specific Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Neuron Specific Enolase (NSE, Enolase-2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Format:
Purified
Formulation:
Antibody in 1X PBS with 0.03% ProClin 300
Immunogen:
A synthetic peptide corresponding to part of the human protein was used as the immunogen for the Neuron Specific Enolase antibody.
Limitation:
This Neuron Specific Enolase antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P09104